Description
- Background: RLBP1 plays a primary role in retinal visual pigment regeneration for both rods and cones in the RPE and sustains M-cone function (including dark adaptation and activity under strong light) in Muller glia, being essential for normal retinal visual cycle and cone photoreceptor function maintenance.
- Gene editing strategy: An icre/ERT2-tdtomato sequence cassette was placed in the mouse Rlbp1 gene under the control of the Rlbp1 promoter.
- Validation: TdTomato expression was observed exclusively in the eye, confirming tissue-specific expression driven by the Rlbp1 promoter in B-Rlbp1-iCreERT2-tdtomato mice.
- Application: The Rlbp1 promoter-driven iCreERT2-tdtomato mouse, B-Rlbp1-iCreERT2-tdtomato mice, enables tamoxifen-inducible, Müller cells-specific Cre recombination, and can achieve precise spatiotemporal control via tamoxifen, high Müller cells specificity (avoiding off-target activity), and utility for lineage tracing, conditional gene manipulation, and studying retinal diseases
Targeting Strategy
Gene targeting strategy for B-Rlbp1-iCreERT2-tdtomato mice.
An icre/ERT2-tdtomato sequence cassette was placed in the mouse Rlbp1 gene under the control of the Rlbp1 promoter.
When B-Rlbp1-iCreERT2-tdtomato mice were crossed with a strain containing a loxP-site flanked sequence of interest, and induced with tamoxifen, Cre-mediated recombination will result in deletion of the floxed sequence in the Müller cells of offspring.
Histopathological Analysis of TdTomato Expression
TdTomato Expression in B-Rlbp1-iCreERT2-tdtomato mice. TdTomato expression was observed exclusively in the eye, confirming tissue-specific expression driven by the Rlbp1 promoter in B-Rlbp1-iCreERT2-tdtomato mice. Scale, 40x.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Rlbp1-iCreERT2-tdtomato mice] (Cat# 113974) was purchased from Biocytogen.
