C57BL/6-Dmp1tm1(icre)Bcgen/Bcgen • 110124
| Product name | B-Dmp1-iCre mice |
|---|---|
| Catalog number | 110124 |
| Strain name | C57BL/6-Dmp1tm1(icre)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | (Mouse) |
| Official symbol | Dmp1dentin matrix protein 1 |
| Chromosome | 5 |
| Aliases | PP; AG1; Dmp; DMP-1 |
| Application | B-Dmp1-iCremicemaybeusefulforgeneratingosteocytesandosteoblasts-specificconditionalmutations |
Homozygousmiceforthetargetedmutationareviable,fertile,normalinsizeanddonotdisplayanygrossphysicalorbehavioralabnormalities
NPY deletion in osteocytes rather than in osteoblasts of Dmp1-iCre (Dmp1-iCre was purchased from Biocytogen Co. Ltd., Beijing, China); Npyfl/fl mice, was confirmed by western blotting for the protein lysates of the isolated osteocytes and osteoblasts (A).
NPY protein expression was much lower in the bone marrow-depleted femur homogenates, but not in the whole bone marrow cells, the whole brain lysate, and the NPY-abundant hypothalamus, of 4-month-old Dmp1-iCre; Npyfl/fl mice than that of Npyfl/fl littermates (C).
Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous mice; WT: samples used for PCR were collected from wild type mice; H2O: blank control.
