• B-Gfap-iCre mice

    C57BL/6-Gfaptm1(icre)Bcgen/Bcgen • 110144

    B-Gfap-iCre mice

    Product nameB-Gfap-iCre mice
    Catalog number110144
    Strain nameC57BL/6-Gfaptm1(icre)Bcgen/Bcgen
    Strain backgroundC57BL/6
    NCBI gene ID (Mouse)
    Official symbolGfap (glialfibrillary acidic protein)
    Chromosome11
    ApplicationThis Gfap-iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of astrocytes cells related signaling.

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    • Description
    • Targeting strategy
    • Phenotypic analysis

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      发表文章

        Description


        This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

        Gene editing strategy
        A target vector was designed to insert iCre-WPRE-PA cassette into mouse endogenous genes 5 'UTR downstream of GFAP. The targeting vector was electroporated into C57BL/6-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulted chimeric mice. These mice were maintained on a C57BL/6 background.
        Phenotype analysis

        In situ hybridization in hippocampal CA1 area to confirm µR selective

        deletion from µRGFAP-/- mice with probes of µR mRNA (red),

        GAD2/ GFAP/vGlut1 mRNA (green), and DAPI nucleus staining (blue).

        * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Gfap-iCre mice] (Cat# 110144) was purchased from Biocytogen.
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